Two-colour two-photon confocal microscopy with isotropic three-dimensional resolution and parallel excitation |
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| Authors: | J. NI&dagger ,L. QIAO,C. WANG,F. ZHAO&dagger ,Y. CHENG,& Z. XU |
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| Affiliation: | State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Science, P.O. Box 800-211, Shanghai 201800, China;State Key Laboratory of Optoelectronic Material and Technologies, Sun Yat-sen University, Guangzhou 510275, China |
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| Abstract: | We demonstrate a novel design of two-colour two-photon fluorescence microscope in which isotropic three-dimensional imaging resolution and high scanning speed can be achieved simultaneously. In our scheme, a three-dimensional optical lattice constructed by multi-beam interference is used for two-colour two-photon fluorescence excitation. Our simulation results show that a resolution of 113.5 nm can be achieved in both transverse and axial directions with two pump pulses at the wavelengths of 400 and 800 nm, respectively; meanwhile, imaging speed can be greatly improved compared with that of traditional two-photon scanning fluorescence microscopes. |
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| Keywords: | Confocal microscopy three-dimensional isotropic resolution two-colour two-photon fluorescence excitation |
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