Prospecting the biofuel potential of new microalgae isolates |
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| Affiliation: | 1. Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan;2. Department of Chemical and Biochemical Engineering, Institute of Geology and Oil-Gas Business Institute Named after K. Turyssov, Satbayev University, Almaty 050043, Kazakhstan;3. Department of Property Protection and Security, Şalpazarı Vocational School, Trabzon University, 61670, Trabzon, Turkey;4. Department of Biology, Karadeniz Technical University, 61080, Trabzon, Turkey;5. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia;6. K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia;7. Faculty of Engineering and Natural Sciences, Bahcesehir University, Istanbul, 34353, Turkey;1. State Key Laboratory of Fluid Power & Mechatronic System, School of Mechanical Engineering, Zhejiang University, Hangzhou, China;2. College of Control Science and Engineering, Zhejiang University, Hangzhou, China;3. Jinhua HydroT Technology Co.Ltd, Jinhua, Zhejiang, China;4. School of Automation and Electrical Engineering, Zhejiang University of Science and Technology, China;1. School of Materials Science and Engineering, Shenyang University of Technology, Shenyang 110870, China;2. School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China;3. Multi-discipline Research Division, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;4. Northwest Institute for Nonferrous Metal Research, Shaanxi Key Laboratory of Biomedical Metal Materials, Xi''an 710016, China;1. College of Chemistry & Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China;2. Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, PR China;1. Key Laboratory of New Opto-Electronic Functional Materials of Henan Province, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, People''s Republic of China;2. Anyang Center for Chemical and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, People''s Republic of China;3. Department of Materials Sciences and Engineering, Islamic Azad University (Saveh Branch), Saveh, 9417764958, Iran |
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| Abstract: | The continued search and urgent need for renewable fuel sources have necessitated the exploration of microalgae to identify relevant species for making biofuels. The aim of the study was bioprospecting and screening native microalgae strains from freshwater habitats of the Almaty region, Kazakhstan, to assess the potential for producing biofuel. The studied strains demonstrated simultaneous biomass productivity, lipid productivity, suitable fatty acid composition, and biodiesel properties. The sequence analysis of the ribosomal DNA internal transcribed spacer partial region and ribulose-bisphosphate carboxylase gene (rbcL) led to the identification of five microalgae: Monoraphidium griffithii ZBD-01, Nephrochlamys subsolitaria ZBD-02, Ankistrodesmus falcatus ZBD-03, Parachlorella kessleri ZBD-04, and Desmodesmus pannonicus ZBD-05. P. kessleri had the highest biomass production (1.42 ± 0.08 g L−1 day−1), lipid productivity (29 ± 1.2 g L−1day−1), and C16–C18 fatty acid contents (90%), followed by A. falcatus and M. griffithi. Gas chromatography/mass spectrometry analysis indicated that the dominant fatty acids in these strains were palmitic, stearic, and oleic acids. The calculated biodiesel properties of P. kessleri and A. falcatus based on fatty acid methyl esters (FAME) profiles showed relatively good fuel properties (cetane numbers - 48 and 50; iodine and saponification values - 83.4 and 103.6 g I₂/100 g oil, 260.8 and 199.5 mg KOH g−1), which correlate well with. Our results suggest that P. kessleri and A. falcatus are promising strains for biodiesel production due to their high lipid productivity, fatty acid profile with relatively high content of oleic acid, and suitable biodiesel properties. The isolated native species of microalgae from natural freshwater bodies of the Almaty region present opportunities for further exploitation for the sustainable production of biomass and biodiesel. |
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| Keywords: | New microalgae Isolation Identification Fatty acids Biodiesel bp, base pair" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" ITS, Internal transcribed spacer rbcL, ribulose-1,5-bisphosphate carboxylase/oxygenase: RUBISCO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" large subunit |
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