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蛹拟青霉发酵菌丝体中虫草酸的提取与测定
引用本文:邵颖,李文,王陶. 蛹拟青霉发酵菌丝体中虫草酸的提取与测定[J]. 食品工业科技, 2012, 0(1): 262-264,267
作者姓名:邵颖  李文  王陶
作者单位:徐州工程学院食品生物工程学院,江苏徐州,221008
基金项目:徐州工程学院校级课题(XKY2007224和XKY2009122)
摘    要:虫草酸,即D-甘露醇,是虫草类真菌中的主要有效成分之一。为更好开发蛹虫草资源,研究了蛹拟青霉发酵菌丝体中虫草酸的提取工艺。依据大量单因素实验以及正交实验得出最佳提取条件为:微波时间80s,微波功率200W,料液比1:100,乙醇浓度70%。在此条件下,对实验室20株蛹拟青霉进行了虫草酸的测定,结果表明22号菌株虫草酸含量高达160.39mg/g,有一定的生产应用价值。

关 键 词:蛹拟青霉  虫草酸  微波提取

Extraction and determination of cordyceps acid in submerged fermentation mycelium of Cordyceps Militaris strains
SHAO Ying,LI Wen,WANG Tao. Extraction and determination of cordyceps acid in submerged fermentation mycelium of Cordyceps Militaris strains[J]. Science and Technology of Food Industry, 2012, 0(1): 262-264,267
Authors:SHAO Ying  LI Wen  WANG Tao
Affiliation:(Food Engineering Department,Xuzhou Insititute of Technology,Xuzhou 221008,China)
Abstract:Cordyceps acid,that is,D-mannitol,is one of the main active ingredients in the type of fungus cordyceps.To explore Cordyceps militaris better,the extracting technology of cordyceps acid in several strains of Cordyceps militaris was determined.According to a large number of single-factor experiments and orthogonal design,the best extraction conditions were drawn:microwave time 80s,microwave power 200W,material-liquid ratio 1:100,ethanol solvent concertration 70%.The strain with the highest acid content among 20 kinds of Cordyceps militaris strains was No.22 strain,whose cordyceps acid content was 160.39mg/g,which had value in production and application.
Keywords:Cordyceps militaris  cordyceps acid  microwave assisted extraction
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