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鳕鱼皮胶原蛋白肽的功能特性及抗氧化活性
引用本文:刘淇,李慧,赵玲,柳倩,曹荣,李亚.鳕鱼皮胶原蛋白肽的功能特性及抗氧化活性[J].食品工业科技,2012(1):135-137,140.
作者姓名:刘淇  李慧  赵玲  柳倩  曹荣  李亚
作者单位:1. 中国水产科学研究院黄海水产研究所,山东青岛,266071
2. 中国水产科学研究院黄海水产研究所,山东青岛266071 青岛大学生物系,山东青岛266071
3. 烟台嘉惠生物科技有限公司,山东烟台,264006
摘    要:以鳕鱼皮胶原蛋白肽为实验材料,研究其吸水性、保水性、乳化性等功能特性,并且进行了体外抗氧化实验。结果表明鳕鱼皮胶原蛋白肽具有较好的吸水性、保水性、吸油性和起泡性,具有一定的乳化性和较弱的泡沫稳定性。体外抗氧化实验结果显示,鳕鱼皮胶原蛋白肽对DPPH自由基、羟自由基和超氧阴离子自由基均有一定的清除效果,且清除率与样品溶液的浓度存在剂量依赖关系;浓度为30mg/mL时,胶原蛋白肽对DPPH·、·OH和O2-·的抑制率分别可达到73.84%、85.76%、64.09%。分级后,分子量1ku以下的胶原蛋白肽对DPPH·和·OH的清除能力最强,30mg/mL时清除率达到84%和90.97%;分子量5ku以上的胶原蛋白肽对O2-·清除能力最强,30mg/mL时清除率达到74.02%。

关 键 词:鳕鱼皮  胶原蛋白肽  功能特性  抗氧化  自由基清除率

Study on functional properties and antioxidation activity of collagen polypeptide from codfish skin
LIU Qi,LI Hui,ZHAO Ling,LIU Qian,CAO Rong,LI Ya.Study on functional properties and antioxidation activity of collagen polypeptide from codfish skin[J].Science and Technology of Food Industry,2012(1):135-137,140.
Authors:LIU Qi  LI Hui  ZHAO Ling  LIU Qian  CAO Rong  LI Ya
Affiliation:1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Biological Department of Qingdao University,Qingdao 266071,China;3.Yantai Jiahui Biotechnology Co.,LTD.,Yantai 264006,China)
Abstract:The functional characteristics and antioxidation activity collagen from codfish skin were studied.Results showed that collagen polypeptide had high water solubility,water adsorption,oil absorption and foaming ability,certain emulsifying property and low foam stability;results of the vitro antioxidant experiment showed:collagen polypeptide from codfish had certain activity to scavenge DPPH·,·OH and O2-·.Further more,scavengeing rate of the sample depended on its dose.The inhibition rate to DPPH·,·OH and O2-· can reach 73.84%,85.76% and 64.09%.After molecular weight grading,vitro antioxidant experiment showed that the molecular weight of component below 1ku works best in clearing DPPH· and ·OH,clearance can reach 84% and 90.97% respectively.The molecular weight of collagen in 5ku above,the clearance rate to O2-· can reach the 74.02%.
Keywords:codfish skin  collagen peptide  functional properties  antioxidation  radical scavenging rate
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