| 酶解辅助反胶束法萃取小麦胚芽蛋白的研究 |
| |
| 引用本文： | 毛丙永,张海华,朱科学.酶解辅助反胶束法萃取小麦胚芽蛋白的研究[J].食品工业科技,2010(3). |
| |
| 作者姓名： | 毛丙永 张海华 朱科学 |
| |
| 作者单位： | 江南大学食品学院,江苏,无锡,214122 |
| |
| 基金项目： | 江苏省自然科学基金(BK2008102); 高等学校博士学科点专项科研基金(20070295034)资助课题 |
| |
| 摘 要： | 采用由AOT(琥珀酸二(2-乙基己基)酯磺酸钠)-异辛烷-KCl缓冲液组成的反胶束体系,联合酶解技术提取小麦胚芽蛋白。探索了酶与底物浓度比、时间、缓冲液pH、温度四个因素对蛋白质前萃率的影响。结果表明:酶解辅助反胶束萃取蛋白是可行的,可以显著提高前萃率,最佳酶与底物浓度比为0.23AU/gPro、时间8h、缓冲液pH8.10、温度60℃。
|
| 关 键 词： | 酶解 反胶束 小麦胚芽蛋白 |
Study on enzymatic extraction of wheat germ protein by reverse micelles |
| |
| MAO Bing-yong,ZHANG Hai-hua,ZHU Ke-xue.Study on enzymatic extraction of wheat germ protein by reverse micelles[J].Science and Technology of Food Industry,2010(3). |
| |
| Authors: | MAO Bing-yong ZHANG Hai-hua ZHU Ke-xue |
| |
| Affiliation: | MAO Bing-yong,ZHANG Hai-hua,ZHU Ke-xue (School of Food Science , Technology,Jiangnan University,Wuxi 214122,China) |
| |
| Abstract: | Reverse micelles combined enzyme hydrolysis was adopted to extract wheat germ protein.Reverse micelles system was formed by sulphosuccinic acid bis(2-ethylhexyl) ester sodium salt(AOT),isooctane and KCl buffer solution.Alcalase was added into the reverse micelles system for assisting protein extraction from defatted wheat germ.Effects of E]/S],extraction time,the pH of buffer solution and temperature on forward extraction efficiency were investigated.Results demonstrated the forward extraction efficiency ... |
| |
| Keywords: | enzymatic reverse micelles wheat germ protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
