工业纤维素酶水解效率的影响因素分析

研究了影响工业纤维素酶Accellerase 1000水解效率的因素,及Accellerase 1000、Spezyme CP和Novozyme 188在不同水解状态下失活情况和葡萄糖对纤维素酶的抑制情况。结果表明反应器类型对水解影响不大,而固含量、酶量、搅拌速率和温度对Accellerase 1000水解效率影响很大。在醋酸缓冲液中,Accellerase 1000的滤纸酶活和纤维二糖酶活失活速率最快;在有木质素存在的醋酸缓冲液中相比各纤维素酶的滤纸酶活失活变慢,但纤维二糖酶失活加快;在滤纸水解过程中,Spezyme CP和Novozyme 188相对较为稳定。葡萄糖对纤维素酶有明显的抑制作用。

Analysis on Effect Factors of Hydrolysis Efficiency of Industrial Cellulases

The factors affecting the efficiency of enzymatic hydrolysis by Accellerase 1000, and the glucose inhibition and deactivation property of several typical industrial cellulases including Accellerase 1000, Spezyme CP, and Novozyme 188 were investigated. The results showed that the hydrolysis efficiency was not significantly affected by the reactor geometry, while the solid content, the cellulase loading, the stirring rate and the temperature had great influence on the hydrolysis efficiency of Accellerase 1000. The activities of filter paper units (FPU) and cellubioase (CBU) of Accellerase 1000 decreased faster in the acetate buffer than Spezyme CP and Novozyme 188. With the existence of lignin particles, the deactivation rate of the filter paper units was decreased, while the deactivation rate of cellubioase activity was increased. The activities of Spezyme CP and Novozyme 188 were more stable than Accellerase 1000. Glucose showed an obvious inhibition effect on the cellulase enzymes.