| LAMP法在阪崎肠杆菌快速检测中的应用 |
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| 引用本文： | 张淑红,吴清平,徐晓可,张菊梅,郭伟鹏. LAMP法在阪崎肠杆菌快速检测中的应用[J]. 食品与机械, 2011, 27(5). DOI: 10.3969/j.issn.1003-5788.2011.05.028 |
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| 作者姓名： | 张淑红 吴清平 徐晓可 张菊梅 郭伟鹏 |
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| 作者单位： | 1. 广东省华南应用微生物重点实验室省部共建国家霞点实验室培育基地,广东广州 510070;广东省菌种保藏与应用重点实验室,广东广州 510070;广东省微生物应用新技术公共实验室,广东广州 510070;广东省微生物研究所,广东广州 510070
2. 广东省微生物研究所,广东广州,510070 |
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| 基金项目： | 广东省科技计划项目(编号:2008A040101001;2009B040500001); 广东省科学院青年科学研究基金项目(编号:qnjj20099) |
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| 摘 要： | 针对阪崎肠杆菌的ompA基因设计4条特异性引物(2条内引物、2条外引物),建立一种快速检测阪崎肠杆菌的DNA环介导恒温扩增法(LAMP)。通过对2株阪崎肠杆菌标准菌株、24株阪崎肠杆菌分离株和18株非阪崎肠杆菌测试,结果表明此方法具有很高的特异性;对其灵敏度进行检验,发现该方法的最低检测限可以达到8CFU/mL,具有较好的敏感性。在1份实验室能力验证盲样和26份实际奶粉样品中应用发现,该方法与传统生化方法结果吻合,具有较好的可靠性。
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| 关 键 词： | 阪崎肠杆菌 ompA基因 环介导恒温扩增方法 |
The application of loop mediated isothermal amplification(LAMP)technique for rapid detection of Ent erob a cter sakazakii |
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| ZHANG Shu-hong,WU Qing-ping,XU Xiao-ke,ZHANG Ju-mei,GUO Wei-peng. The application of loop mediated isothermal amplification(LAMP)technique for rapid detection of Ent erob a cter sakazakii[J]. Food and Machinery, 2011, 27(5). DOI: 10.3969/j.issn.1003-5788.2011.05.028 |
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| Authors: | ZHANG Shu-hong WU Qing-ping XU Xiao-ke ZHANG Ju-mei GUO Wei-peng |
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| Affiliation: | ZHANG Shu-hong1,2,3,4 WU Qing-ping4 XU Xiao-ke1,4 ZHANG Ju-mei4 GUO Wei-peng4(1.State Key Laboratory of Applied Microbiology(Ministry-Guangdong Province Jointly Breeding Base),Guangzhou,Guangdong510070,China,2.Guangdong Provincial Key Laboratory of Microbial Culture Collection andApplication,3.Guangdong Provincial Public Laboratory forApplied and New Technology of Microbiology,4.Guangdong Institute of Microbiology,... |
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| Abstract: | Four primers including two inner primers,two outer prim-ers were designed specifically to recognize the ompA gene of E.sakazakii and a method of loop-mediated isothermal amplification was set up to detect Enterobacter sakazakii rapidly.The results sug-gested that the method had high speciality to 2 E.sakazakii typical strains,24isolates and 18non-E.sakazakii.More over,it had good sensitivity with the lowest detectable limit of 8CFU/mL.Through detecting the proficiency tests of E.sakazakii in milk pow-der an... |
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| Keywords: | Enterobacter sakazakii ompAgene loop mediated isothermal amplification |
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