| 抗噬菌体菌株荧光假单胞菌A46在D-异抗坏血酸钠工业生产中的应用研究 |
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| 引用本文： | 孙文敬,赵峰梅,黄惠英,杨庆文,郭金权,蒋明珠.抗噬菌体菌株荧光假单胞菌A46在D-异抗坏血酸钠工业生产中的应用研究[J].食品与发酵工业,2001,27(7):8-11. |
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| 作者姓名： | 孙文敬 赵峰梅 黄惠英 杨庆文 郭金权 蒋明珠 |
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| 作者单位： | 1. 山西省生物研究所
2. 郑州市生物化工厂 |
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| 摘 要： | 荧光假单胞菌A4 6是通过紫外线诱变K10 0 5菌株所得到的一株抗噬菌体菌株。在噬菌体存在的情况下 ,使用菌株A4 6和K10 0 5在 50kL的发酵罐上进行了 2 酮基 D 葡萄糖酸的发酵生产。结果表明 ,荧光假单胞菌A4 6对噬菌体的抗性稳定 ,其发酵周期短 ,发酵转化率高 ;经过多次传代 ,荧光假单胞菌A4 6对噬菌体的抗性及其发酵生产 2 酮基 D 葡萄糖酸的能力没有改变 ;该菌株的发酵产物可以用于D 异抗坏血酸钠的合成中
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| 关 键 词： | 2酮基D葡萄糖酸 荧光假单胞菌 噬菌体 抗噬菌体菌株 D异抗坏血酸钠 |
Study on Application of Phage - resistant Strain Pseudo monas fluorescens A46 in the Industrial Production of Sodium D-isoascorbate |
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| Sun Wenjing,Zhao Fengmei,Huang Huiying,Yang Qingwen,Guo Jinquan,Jiang Mingzhu.Study on Application of Phage - resistant Strain Pseudo monas fluorescens A46 in the Industrial Production of Sodium D-isoascorbate[J].Food and Fermentation Industries,2001,27(7):8-11. |
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| Authors: | Sun Wenjing Zhao Fengmei Huang Huiying Yang Qingwen Guo Jinquan Jiang Mingzhu |
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| Affiliation: | Sun Wenjing 1 Zhao Fengmei 1 Huang Huiying 2 Yang Qingwen 1 Guo Jinquan 1 Jiang Mingzhu 1 1 |
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| Abstract: | Pseudomonas fluorescens A46 was a phage resistant strain obtained from the mutation of the strain K1005 by using U.V. light as a mutagen. In the presence of phages, 2 keto D gluconic acid fermentation by Pseudomonas fluorescens A46 and its parent in 50?kL fermentor was studied. The results showed that Pseudomonas fluorescens A46 was indeed a phage resistant strain and the resistance was stable, with shorter fermentation period and higher fermentation conversion rate than its parent. After 17generation, its resistance against phages and 2 keto D gluconic acid producing ability had not changed. The fermentation product of Pseudomonas fluorescens A46 could be used in the synthesis of sodium D isoascorbate. |
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| Keywords: | keto D gluconic acid Pseudomonas fluorescens phage phage resistant strain sodium D isoascorbate |
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