| 响应面分析法优化羊血中SOD的热变性提纯工艺条件 |
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| 引用本文： | 于颖,王小丽,徐桂花. 响应面分析法优化羊血中SOD的热变性提纯工艺条件[J]. 肉类研究, 2009, 0(11): 25-30 |
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| 作者姓名： | 于颖 王小丽 徐桂花 |
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| 作者单位： | 宁夏大学农学院食品系,宁夏,银川,750021;宁夏农垦事业管理局,宁夏,银川,750001 |
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| 摘 要： | 利用响应面分析法(Response Surface Methodology)对羊血中SOD的热变性提纯工艺进行优化。在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用3因素水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以SOD的比活力为响应值作响应面和等高线。在分析各个因素的显著性和交互作用后,得出最佳工艺条件为:热变性温度55℃;热变性时间15min;0.01mol/lCuCl2的添加量2%。在最佳工艺条件下,SOD活性为1072.43U/mg蛋白。
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| 关 键 词： | 羊血 热变性提取 响应面分析法 优化 |
Study on the Heating Denatureation Extraction of SOD from Sheep Blood with Response Surface Analysis |
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| YU Ying,WANG Xiaoli,XU Guihua. Study on the Heating Denatureation Extraction of SOD from Sheep Blood with Response Surface Analysis[J]. Meat Research, 2009, 0(11): 25-30 |
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| Authors: | YU Ying WANG Xiaoli XU Guihua |
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| Abstract: | Based on single factor tests,the optimum heating denatureation extraction conditions of SOD from sheep blood were obtained through Box-benhnken central combination design and RSM.The results showed that the optimum heating denatureation extraction conditions of SOD are as follows:heating denatureation temperature 55℃,heating denatureation time 15min and 2%.Under such conditions,the activity exceeds 3 000 U/mg protein was reached. |
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| Keywords: | sheep blood heating denatureation extraction response surface methodology optimization |
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