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| 微囊藻毒素LR、RR的提取和纯化 | |
| 引用本文： | 卫涛,向铮,张婧婧,冯小刚. 微囊藻毒素LR、RR的提取和纯化[J]. 江苏化工, 2007, 35(5): 19-22 |
| 作者姓名： | 卫涛 向铮 张婧婧 冯小刚 |
| 作者单位： | 1. 温州医学院药学院化学教研室,浙江,温州,325035 2. 南京理工大学化工学院,江苏,南京,210094 3. 东南大学纳米光催化研究中心,江苏,南京,210096 |
| 基金项目： | 国家高技术研究发展计划资助(2002AA304302) |
| 摘 要： | 以天然水华蓝藻为原料,建立了以甲醇溶液提取和固相萃取为主要步骤的微囊藻毒素分离纯化方法。比较不同极性提取剂对微囊藻毒素的提取效果,结果表明:60%甲醇有较高的提取效率,同时优化了提取方法。并通过调节溶液pH值至藻胆蛋白等电点的方法除去共提取的大量藻胆蛋白,取得了很好的净化效果,为微囊藻毒素的分离和纯化研究提供了基础。 |
| 关 键 词： | 微囊藻毒素 提取 纯化 固相萃取 |
| 文章编号： | 1002-1116(2007)05-0019-04 |
| 修稿时间： | 2007-07-04 |
Extraction and Purification of Microcystins-LR, RR |
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| WEI Tao,XIANG Zheng,ZHANG Jing-jing,FENG Xiao-gang. Extraction and Purification of Microcystins-LR, RR[J]. Jiangsu Chemical Industry, 2007, 35(5): 19-22 | |
| Authors: | WEI Tao XIANG Zheng ZHANG Jing-jing FENG Xiao-gang |
| Abstract: | Some new methods were established to isolate and purify Microcystins from natural cyanobacterial bloom,by the main steps of extraction with aqueous methanol and solid phase extraction.The optimized method was evaluated with different solvents applied to assess the efficiency of microcystins extraction.60% methanol had the highest efficiency,and the massive of phycobiliprotein was removed by adjusting the pH of solvents to the isoelectric point.This result provided a solid basis for obtaining pure microcystins efficiently. |
| Keywords: | microcystins extraction purifying SPE |
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