重组小分子多肽的SDS-PAGE分析方法

比较了小分子多肽SDS PAGE的各种显色方法 ,结果表明 ,考马斯亮蓝R 2 5 0染色需要 3μg以上的样品 ,相对分子质量 30 0 0左右的肽才能得到清晰条带 ,而对含量低的基因工程产物不能显色 .银染可用于检测微量的分子多肽 ,银染过程中用戊二醛和甲醛进行敏化而减少固定时间 ,可显著提高检测灵敏度 ,是检测微量基因工程产物的好方法

Analysis of Low Molecular Peptides by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electophoresis

This article described several blotting methods of SDS polycrylamide gel electrophoresis to analysis low molecular weight polypeptides. The result showed that when the coomassie brilliant G 250 was used to stain the gel, the band of the peptide with 3 000 molecular weight couldn't be seen clearly unless its mass is above 3 microgramme. During the process of silver blotting the Glutaraldenyde and the Formaldehyde were used to sensitize the stain process, to reduce the time to fix the polypeptides. The sensitivity was significantly improved to detect low molecular peptides by this method. Thus, silver blotting is anexcellent way to detect the tiny amount low molecular peptides produced by recombined microorganism.