Au/PANA/PVAc and Au/P(ANA-co-CNTA)/PVAc electrospun nanofibers as tyrosinase immobilization supports |
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| Authors: | Rana Golshaei Zeliha Guler Gokce Sayed Mehdi Ghoreishi |
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| Affiliation: | 1. University of Kashan, Institute of Nano Science and Nano Technology, Kashan, I. R. Iran;2. Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Maslak, Turkey;3. Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, I. R. Iran |
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| Abstract: | Au/poly anthranilic acid/poly vinyl acetate and Au/poly(anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline)/poly vinyl acetate nanofibers through electrospinning and their modification with covalent tyrosinase (Ty) immobilization was performed. It was realized by surface activation using N-(3-dimetylaminopropyl)-N-ethylcarbodimide hydrochloride/N-hydroxysuccinimide chemistry. Electrochemical impedance spectroscopy (EIS), FTIR–ATR, Raman spectroscopy, and bicinchoninic acid assay analyses demonstrated that Ty was stably and covalently bonded onto the nanofibers. Increase in surface roughness [atomic force microscopy (AFM)] and the presence of Cu atoms in the nanofiber composition after enzyme immobilization confirmed the Ty immobilization. The charge transfer resistances of the nanofibers decreased due to changes in the nanofiber surfaces after attachment of enzyme. |
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| Keywords: | Atomic force microscopy covalent immobilization electrochemical impedance spectroscopy tyrosinase |
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