Biomimetic hydroxyapatite coating on glass coverslips for the assay of osteoclast activity in vitro |
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| Authors: | Asiri K A R Wijenayaka Christopher B Colby Gerald J Atkins Peter Majewski |
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| Affiliation: | (1) Bone Cell Biology Group, Discipline of Orthopaedics and Trauma, University of Adelaide, Adelaide, SA, 5005, Australia;(2) School of Advanced Manufacturing and Mechanical Engineering/Mawson Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia;(3) School of Chemical Engineering, University of Adelaide, Adelaide, SA, 5005, Australia |
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| Abstract: | The osteoclast (OC) is the cell type responsible for the resorption of bone. The activity of this cell is important in the
aetiology of a large number of skeletal pathologies, and also for the biocompatibility and osseointegration of orthopaedic
implant materials. OC mediated acid hydrolysis of calcium phosphate from the bone matrix offers a prime means of studying
the biology and activity of this cell type. We have developed a method of coating glass coverslips with a hydroxyapatite (HA)-like
mineral, using a biomimetic approach. Hydroxylation followed by formation of a self assembled monolayer (SAM) using the surfactant
triethoxysilylpropyl succinic anhydride (TESPSA), allowed biomimetic deposition of HA-like mineral from a simulated body fluid
(SBF). The biocompatibility of the TESPSA SAM-HA coated glass coverslips was tested by culturing human mature OC present in
samples of giant cell tumour of bone (GCT). Parameters of OC activity were assayed, including F-actin ring formation, release
of calcium and formation of osteoclastic resorption pits, confirming that OC were able to attach to and resorb the coated
surface. This approach for the preparation of HA coatings on glass coverslips could have wide applicability for the study
of osteoclast behaviour in vitro.
Gerald J. Atkins and Peter Majewski share senior author status. |
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