| 快速溶剂萃取法在灵芝多糖提取中的应用 |
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| 引用本文： | 王宇晴,牛雯颖,刘松梅,郑春英.快速溶剂萃取法在灵芝多糖提取中的应用[J].中国调味品,2020(2):107-111. |
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| 作者姓名： | 王宇晴 牛雯颖 刘松梅 郑春英 |
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| 作者单位： | 黑龙江大学农业微生物技术教育部工程研究中心;黑龙江大学生命科学学院黑龙江省普通高校微生物重点实验室 |
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| 摘 要： | 以灵芝子实体为原料,采用单因素及正交试验系统研究快速溶剂萃取法提取灵芝多糖的工艺条件,同时采用高效毛细管电泳法分析灵芝多糖的单糖组成成分。结果显示:快速溶剂萃取法提取灵芝多糖的最佳工艺参数为提取温度110℃,提取压力90Pa,提取时间20min,循环2次。单糖组成分析表明灵芝多糖中含葡萄糖、甘露糖和半乳糖的物质的量之比为2.8063∶1.0000∶2.1910。
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| 关 键 词： | 快速溶剂萃取法 灵芝多糖 工艺优化 单糖组成 |
Application of Fast Solvent Extraction Method in the Extraction of Polysaccharide from Ganoderma lucidum |
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| WANG Yu-qing,NIU Wen-ying,LIU Song-mei,ZHENG Chun-ying.Application of Fast Solvent Extraction Method in the Extraction of Polysaccharide from Ganoderma lucidum[J].China Condiment,2020(2):107-111. |
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| Authors: | WANG Yu-qing NIU Wen-ying LIU Song-mei ZHENG Chun-ying |
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| Affiliation: | (Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China;Key Laboratory of Microbiology of Regular Institutions of Higher Learning in Heilongjiang Province,College of Life Sciences,Heilongjiang University,Harbin 150080,China) |
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| Abstract: | Using Ganoderma lucidum fruit body as the raw material,single factor and orthogonal experiments are used to study the process conditions for extracting polysaccharides fromGanoderma lucidum by fast solvent extraction method systematically,and the monosaccharide components of Ganoderma lucidum polysaccharides are analyzed by high performance capillary electrophoresis.The results show that the best extraction parameters are as follows:extraction temperature is 110 ℃,extraction pressure is 90 Pa,extraction time is 20 min,the times of cycle is 2.Monosaccharide composition analysis shows that the ratio of glucose,mannose and galactose in Ganoderma lucidum polysaccharides is2.8063∶1.0000∶2.1910. |
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| Keywords: | ASE Ganoderma lucidumpolysaccharides process optimization monosaccharide composition |
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