乳酸菌胞外多糖的纯化及对小鼠血清和肝组织抗氧化性的影响

本实验对乳酸乳球菌乳亚种胞外多糖的纯化工艺及其抗氧化活性进行研究。纯化结果表明，选择DA201-C树脂，调节胞外多糖的pH值为3.5，在45℃条件下，树脂用量为2%(V/V)，静态吸附20h后糖保留率为81.56%，蛋白脱除率为72.09%，脱色率为81.69%。通过动物实验，检测小鼠血清和肝组织中的过氧化氢酶(CAT)活力、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力可知，灌胃胞外多糖组的小鼠与对照组小鼠相比，抗氧化能力显著性提高。

Study on Separation and Purification of Extracellular Polysaccharide in Fermentation Broth of Lactococcus lactis subsp. lactis and Its Effects on Antioxidant Functions of Mouse Serum and Liver

In this study, the process conditions were investigated for purifying extracellular polysaccharide from pretreated fermentation broth (extracellular polysaccharide solution) of Lactococcus lactis subsp. lactis with macroporous resin, and the extracellular polysaccharide was administered to ICR mice by gavage to investigate its antioxidant activity. The results showed that under the conditions of adsorbing the extracellular polysaccharide solution at pH 3.5 with resin DA201-C of 2% for 20 h at 45 ℃ in static state, the reservation ratio, protein removal ratio and de-colorization ratio are 81.56%, 72.09% and 81.69%, respectively. Moreover, the animal experiment indicated that compared with the control, the antioxidant functions of serum and liver in extracellular polysaccharide-administered mice are significantly improved through enhancing CAT and SOD activities and reducing MDA contents in mouse serum and liver tissue.