乳源性促矿物元素吸收肽超滤技术的优化

采用超滤技术，对通过胰蛋白酶酶解制备的粗肽进行初步分离纯化，从而获得较高纯度的促矿物元素吸收肽。对超滤工艺中几个主要技术参数(超滤压力、料液初始稀释倍数、料液pH 值、料液温度)进行了研究，以膜通量、目标肽回收率和P/N(摩尔比)作为其结果的评价指标。结果表明：利用截留分子量为6000D 的中空纤维超滤膜，在超滤压力为0.125MPa、料液初始稀释倍数为4、料液pH5.5、料液温度45℃的条件下，以45min 作为一个超滤周期，能得到纯度最高和活性最大的促矿物元素吸收肽。该条件下获得的目标肽可以将NaH2PO4 和CaCl2 在体外产生沉淀的时间推迟15min，此时目标肽回收率为84.1%。

Optimization of Ultra-filtration Technology of Milk-derived Mental-binding Peptides

This study aimed to simply purify mental-binding peptides which were derived from hydrolyzed whey protein by ultra-filtration technology. The main technical parameters were studied, including ultra-filtration pressure, dilution multiple of liquid material, its pH value and temperature, taking membrane flux, peptide recovery rate and initial molar ratio of P to N as evaluation indexes. The results indicated that high-purity and high-activity mental-binding peptides can be obtained a ultrafiltration circle of 45 min with the ultra-filtration membrane of 6000 D molecular weight cut off under the conditions of ultra-filtration pressure 0.125 MPa, dilution multiple of liquid material 4, its pH 5.5 and temperature 45 ℃. Under these conditions, the appearance time of precipitates can be postponed for 15 min, which is created from the reaction between NaH2PO4 and CaCl2. Meanwhile, the peptide recovery rate is 84.10%.