吖啶橙-曙红Y能量转移荧光猝灭法测定痕量镍

在pH5.0溶液中，十二烷基硫酸钠(SLS)作用下，吖啶橙与曙红Y能发生有效的能量转移，使曙红Y荧光增强，Ni2+的加入使曙红Y在541nm处发生荧光猝灭，其荧光猝灭程度与镍的含量呈线性关系，由此建立了能量转移荧光测定痕量镍的新方法。实验表明，镍含量在0.001～0.1μg/ml范围内与荧光猝灭强度△F符合比尔定律，检出限为0.53ng/ml，对1μg Ni2+进行11次测定，其相对标准偏差为2.4% 。用本法测定植物样中镍含量，相对误差小于3%，结果满足痕量分析要求。

Determination of Trace Nickel by Acridine Orange-Eosin Y Energy-transfer Fluorescence-quenching Method

n the aqueous medium at pH 5.0 and in the presence of sodium dodecyl sulfate (SLS), the energy transfer from acridine orange (AO) to eosin Y(EY) enhances the fluorescence of EY, and the addition of nickel (II) makes EY occur fluorescence quenching at 541 nm. The degree of quenching and the content of nickel is linear. A new method for determine trace nickel based on energy transfer was established. The results showed that when the content of nickel is in the range of 0.001 to 0.1 μg/ml, its relationship with the intension of fluorescence quenching (△F) fits to Bill law, and its detection limit is 0.53 ng/ml . For 11 times determination of 1 μg standard nickel (II), the relative standard deviation is 2.4%. To determine the content of nickel in plant samples, the relative error is less than 3%, which meets the requirement of trace analysis.