黏红酵母离子注入诱变及其发酵产生β- 胡萝卜素条件的研究

利用低能N+ 注入黏红酵母高压突变株G-39，经筛选获得高产β- 胡萝卜素的离子诱变株GL-5，其β- 胡萝卜素产量由出发菌株的9.64mg/L 提高到17.36mg/L，增加了80.08%。通过均匀设计试验法，初步确定了诱变株GL-5 的β- 胡萝卜素发酵最适条件(葡萄糖40g/L、蛋白胨30g/L、酵母膏10g/L、番茄汁3ml/L、核黄素0.5mg/L、初始pH6.0、摇瓶装量50ml/250ml、接种量50ml/L)，使其β- 胡萝卜素产量进一步由17.36mg/L 提高到34.21mg/L，比对照增加了98.09%。这表明，离子诱变株GL-5 是一株优良的高产β- 胡萝卜素突变株，离子注入对该菌种具有良好的诱变效果。

Mutation Effect of Ion Implantation on Rhodotorula glutinis and Its Fermentation Conditions for β-Carotene Production

A new mutant strain GL-5 was obtained from the high-pressure mutant strain G-39 of Rhodotorula glutinis by N+ ion implantation. Its β-carotene yield is up to 17.36mg/L, higher 80.08% than that of the initial strain G-39. Its optimum medium (glucose 40 g/L, peptone 30 g/L, yeast extract 10 g/L, tomato extract 3 ml/L, riboflavin 0.5 ml/L, inoculation amount 50 ml/L, culture broth quantity 50 ml in 250-ml shake flask and initial pH 6.0) for producing β-carotene was obtained by uniform design experiment. Under the above culture conditions, the yield of β-carotene produced by the mutant strain GL-5 reaches 34.21 mg/L, higher 98. 09% than the control. This study suggested that the mutant strain GL-5 is an excellent strain for producingβ-carotene and has bright application prospect in biochemical engineering.