Interactions of a vesicular stomatitis virus G protein fragment with phosphatidylserine: NMR and fluorescence studies

Closed-loop recycling with periodic intra-profile injection (CLRPIPI) is a binary chromatographic separation technique. It is similar to simulated moving bed chromatography in that sample is injected into the interior of the circulating chromatographic profile. Although the CLRPIPI process is repetitive, it is different from SMB in that CLRPIPI is not continuous. It is shown that the CLRPIPI process reaches a steady state and that high purity fractions can be collected.