| 大豆卵磷脂中脑苷脂类物质的分析与检测研究 |
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| 引用本文： | 韩雪,汪勇,胡长鹰,王丽丽,刘元法,王兴国.大豆卵磷脂中脑苷脂类物质的分析与检测研究[J].中国粮油学报,2011,26(5):111-118. |
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| 作者姓名： | 韩雪 汪勇 胡长鹰 王丽丽 刘元法 王兴国 |
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| 作者单位： | 1. 暨南大学食品科学与工程系,广州,510632
2. 江南大学食品学院,无锡,214122 |
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| 基金项目： | "863"计划，中央高校基本科研业务费专项资金 |
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| 摘 要： | 通过电喷雾质谱(ESI/MS)和高效液相色谱-蒸发光散射器(HPLC-ELSD)鉴定和分析用柱层析法纯化大豆卵磷脂得到的各组分主要成分。经质谱检测纯化的组分中主要有两种分子类型的神经酰胺,分别是C18:0 d18:1Cer和C25:0 h d18:1Cer;主要有两种分子类型的脑苷脂,分别是C16:0 t18:2Ce和C17:0Ce。通过HPLC-ELSD分析,二次柱层析得到的神经酰胺含量99.43%,回收率为98.23%;脑苷脂含量为98.86%,回收率为97.27%。
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| 关 键 词： | 脑苷脂 神经酰胺 电喷雾质谱 HPLC-ELSD |
Study on Analysis and Detection of Cerebrosides from Soybean Lecithin |
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| Han Xue,Wang Yong,Hu Changying,Wang Lili,Liu Yuanfa,Wang Xingguo.Study on Analysis and Detection of Cerebrosides from Soybean Lecithin[J].Journal of the Chinese Cereals and Oils Association,2011,26(5):111-118. |
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| Authors: | Han Xue Wang Yong Hu Changying Wang Lili Liu Yuanfa Wang Xingguo |
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| Affiliation: | Han Xue1 Wang Yong1 Hu Changying1 Wang Lili1 Liu Yuanfa2 Wang Xingguo2(Department of Food Science and Engineering,Jinan University1,Guangzhou 510632)(School of Food Science and Technology,Jiangnan University2,Wuxi 214122) |
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| Abstract: | The composition of portions purified from soy lecithin by silica gel column chromatography(SGCC) was analyzed using ESI/MS(electrospray ionization mass spectrometry) and HPLC-ELSD(evaporative light scattering detector).Two molecular species of ceramide(Cer)(C18:0 d18:1Cer and C25:0h d18:1Cer) and two molecular species of cerebroside(CE)(C16:0 t18:2Ce and C17:0Ce) were identified by ESI/MS in the portions.A content of 99.43% with a recovery of 98.23% and a content of 98.86% with a recovery of 97.27% were det... |
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| Keywords: | cerebroside ceramides ESI/MS HPLC-ELSD |
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