Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus) |
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Authors: | Yu-Ru Huang Chyuan-Yuan Shiau Hui-Huang Chen Bi-Chin Huang |
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Affiliation: | 1. Department of Food Science, National Penghu University, Penghu 880, Taiwan, ROC;2. Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, ROC;3. Department of Food Science, National Ilan University, Ilan, Taiwan, ROC |
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Abstract: | Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were successfully extracted from the skin of balloon fish (Diodon holocanthus) with yields of 4% and 19.5% respectively (based on dry weight). According to the electrophoretic patterns, both the ASC and PSC consisted of two different α chains (α1 and α2), were characterized to be type I, and contained imino acid of 179 and 175 residues/1000 residues, respectively. The PSC had a lower content of high-molecular weight cross-links than the ASC. The ultraviolet (UV) absorption spectrum of collagens showed that the distinct absorption was between 210 and 230 nm. A maximum solubility in 0.5 M acetic acid was observed at pH 1–5, and a sharp decrease in solubility above 4% (w/v) in both the ASC and PSC was observed in the presence of NaCl. The denaturation temperatures (Td) of the ASC and PSC measured by viscometry were 29.01 °C and 30.01 °C, respectively. The maximum temperatures (Tmax) of the ASC and PSC were 29.64 °C and 30.30 °C, respectively. |
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Keywords: | Balloon fish Acid-solubilized collagen Pepsin-solubilized collagen Denaturation temperature Solubility |
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