卵叶娃儿藤生物碱对宫颈癌细胞株HeLa的体外作用

为了观察卵叶娃儿藤总生物碱Tylophora ovata(Lindl.)Hook.ex Steud,alkaloids TOHa]体外抑制人宫颈癌细胞株HeLa增殖、诱导细胞凋亡的作用,将不同浓度的TOHa与HeLa细胞在体外培养,用MTT法观察TOHa对HeLa细胞的生长抑制作用,测定其在490nm的OD值;用细胞形态学、AnnexinV/PI双标记检测细胞凋亡。结果表明,TOHa能抑制HeLa细胞的增殖和活力,呈现作用时间和剂量的依赖关系。HeLa细胞经TOHa作用后,Annexin V /PI-表达升高,Giemsa染色后出现凋亡细胞的特征性改变。

Effects of Tylophora ovata (Lindl) Hook. ex Steud Alkaloids (TOHa) on Proliferation and Apoptosis of HeLa Cells in vitro

This study aimed to explore the effect of Tylophora ovata(Lindl.) Hook.ex Steud(TOHa) alkaloids on proliferation and apoptosis of HeLa cells in vitro.Different concentrations of TOHa were used to treat HeLa cells in vitro for different time.The proliferation was determined by MTT assay,and the cell apoptosis was determined by cell morphology,Annexin V/PI and Giemsa labeling method.It was found experimentally that the TOHa can inhibit the proliferation and activity of Hela cells,and the inhibition effects depend on reaction time and TOHa dose.After being treated with TOHa,Annexin V /PI-expression of Hela cells rises,and their apoptosis features change accroding to the results of Giemsa staining.