氯苯降解菌的筛选及降解条件

以筛选氯苯降解菌株为目的从抚顺石油二厂污水处理曝气池中的活性污泥中筛选到一株具有降解氯苯能力的菌株, 命名为LP01, 依据该菌株的菌落特征、菌体形态以及染色反应和生理生化反应鉴定, 初步判断其属于假单孢菌属(Pseudomonas .sp)。同时分别考察培养温度、氯苯质量浓度、pH 值及摇床转速各单因素对该菌株降解氯苯性能的影响。并设计正交实验以选择菌株LP01 对氯苯降解的最佳条件。实验结果表明, LP01菌株对氯苯降解的最佳条件为:培养温度为35 ℃, 底物质量浓度为30 mg/ L, pH 值为8, 摇床转速为120 r/min 。在该条件下该菌株对氯苯的降解率可达到93.9%。

Isolation of a Stain of Degrading Chlorobenzene and Its Degrading Conditions

In order to have the strain capable of degrading chlorobenzene, a strain LP01 was isolated from Fushun No.2 refinery's sewage disposal factory activated sludge. The stain is identified as Pseudomonas sp. by the appraisement of colonies character, thallus morphology, staining and recessive reaction. The factors influencing the strain's chlorobenzene degrading ability, such as cultivatable temperature, substrate mass concentration, pH, and shaking table revolution were studied. The optimum conditions for degrading chlorobenze were chosen by orthogonal experiments. The results show that the optimum conditions are as follows: temperature is 35 ℃, mass concentration of substrate is 30 mg/L, pH is 8, shaking table revolution is 120 r/min, under which the biodegrading rate reaches to 93.9%.