| 猫棒束孢真菌液体发酵工艺研究 |
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| 引用本文： | 杨喜花,张生万,任连生.猫棒束孢真菌液体发酵工艺研究[J].中国酿造,2012(11):122-124. |
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| 作者姓名： | 杨喜花 张生万 任连生 |
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| 作者单位： | [1]山西大学生命科学学院,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]山西省肿瘤研究所比较医学部,山西太原030013 |
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| 基金项目： | 山西省实验动物专项基金（2012（K05）） |
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| 摘 要： | 经过对培养基、装量、温度、发酵时间等因素的考察,确定了猫棒束孢最佳发酵工艺:培养基为蛋白胨0.6%,酵母膏1.2%,蔗糖2.4%,硫酸镁0.05%,磷酸二氢钾0.1%,用饱和碳酸氢钠溶液调整pH值为6.5~7.0;培养基装量为500mL;发酵温度为26.5℃;发酵时间为48h。
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| 关 键 词： | 猫棒束孢 真菌 液体发酵 |
Optimization of submerged fermentation conditions of Isaria Felina |
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| YANG Xihua,ZHANG Shengwan,REN Liansheng.Optimization of submerged fermentation conditions of Isaria Felina[J].China Brewing,2012(11):122-124. |
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| Authors: | YANG Xihua ZHANG Shengwan REN Liansheng |
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| Affiliation: | 1.College of Life Science, Shanxi University, Taiyuan 030006, China; 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China; 3. Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China) |
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| Abstract: | Effect of culture medium, culture medium volume, culture temperature and culture time on mycelia dry weight of Isaria felina was studied. The optimum medium was as followed： peptone 0.6%, yeast extract 1.2%, sucrose 2.4%, MgSO4 0.05%, KH2PO4 0.1%, pH 6.5-7.0 adjusted by satu- rated solution of NaHCO3, medium volume 500ml/3000ml shaking flask, culture temperature 26.5℃ and culture time 48h. |
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| Keywords: | Isaria felina fungus submerged fermentation |
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