| L.paracasei HD1-7与S.albulus1-25原生质体融合选育产天然防腐剂的优良菌株 |
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| 引用本文： | 雷虹,张铁丹,金忠斌,庄海霁,何欣,张基亮,平文祥,周东坡.L.paracasei HD1-7与S.albulus1-25原生质体融合选育产天然防腐剂的优良菌株[J].食品工业科技,2011(1):146-150,155. |
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| 作者姓名： | 雷虹 张铁丹 金忠斌 庄海霁 何欣 张基亮 平文祥 周东坡 |
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| 作者单位： | 黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,黑龙江哈尔滨,150080 |
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| 基金项目： | 黑龙江省科技厅重大攻关项目(GA07B401); 黑龙江省科技厅重大攻关项目(GB05B401); 黑龙江省科技厅青年基金项目(QC03C24); 哈尔滨市科技局科技攻关项目(2009AA3CN079) |
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| 摘 要： | 为获得更具优良应用特性的微生物源天然防腐剂,采用原生质体融合技术,以Lactobacillus paracasei HD1-7和Streptomyces albulus1-25为亲株选育优良菌株,研究了L.paracasei HD1-7和S.albulus1-25原生质体制备与再生条件,通过表型、抑菌谱、抗药性和总DNA含量比较等手段筛选鉴定融合子,最终选育出3株以L.paracasei HD1-7为受体、获得来自S.albulus1-25的抑酵母菌基因的融合子。
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| 关 键 词： | L.paracasei HD1-7 S.albulus1-25 天然防腐剂 原生质体制备 融合子鉴定 |
Breeding strains of excellent biopreservative by Lparacasei HD1-7 and S.albulus 1-25 protoplast fusion |
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| LEI Hong,ZHANG Tie-dan,JIN Zhong-bin,ZHUANG Hai-ji,HE Xin,ZHANG Ji-liang,PING Wen-xiang,ZHOU Dong-po.Breeding strains of excellent biopreservative by Lparacasei HD1-7 and S.albulus 1-25 protoplast fusion[J].Science and Technology of Food Industry,2011(1):146-150,155. |
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| Authors: | LEI Hong ZHANG Tie-dan JIN Zhong-bin ZHUANG Hai-ji HE Xin ZHANG Ji-liang PING Wen-xiang ZHOU Dong-po |
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| Affiliation: | LEI Hong,ZHANG Tie-dan,JIN Zhong-bin,ZHUANG Hai-ji,HE Xin,ZHANG Ji-liang,PING Wen-xiang,ZHOU Dong-po(Key Laboratory of Microbial,College of Life Science,Heilongjiang University,Harbin 150080,China) |
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| Abstract: | In order to obtain microbial preservative with excellent applicability,L.paracasei HD1-7 and S. albulus 1-25 were used as parents to construct strains through protoplast fusion technique. Optimal protoplast fusion conditions were systematically investigated. Through phenotype characteristic,antibacterial spectrum,drug resistance property and DNA content,3 optimal fusants were identified. The fusants had the gene of inhibiting yeast from S. albulus 1-25. |
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| Keywords: | L paracasei HD1-7 S albulus 1-25 biopreservative protoplast fusion fusant identification |
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