黄蜀葵胶中的糖分析

目的：分析不同来源的黄蜀葵胶中的糖含量、多糖相对分子质量及其单糖组成。方法：采用3,5-二硝基水杨酸比色法测定4种黄蜀葵胶中还原糖、总糖和总多糖的含量，并解析其红外吸收光谱图；凝胶渗透色谱法测定纯化后的4种多糖的相对分子质量，气相色谱法分析其单糖组成。结果：4种黄蜀葵胶的还原糖质量分数分别为51.07%、9.00%、53.94%和11.46%，总糖质量分数分别为79.95%、78.99%、91.98%和61.13%，总多糖质量分数分别为28.88%、69.99%、38.04%和49.67%；红外谱图显示AMG-1、AMG-3中主要含α-D-吡喃葡萄糖，AMG-2和AMG-4中主要含有甘露糖；4种黄蜀葵胶中多糖的峰值相对分子质量分别为3.91×103、5.36×105、3.87×103和5.12×105；4种样品均含有甘露糖、葡萄糖和半乳糖，其物质量之比分别为0.42:1.00:0.27、1.00:0.15:0.54、0.27:1.00:0.15和1.00:0.12:0.48。结论：不同来源的黄蜀葵胶在还原糖、总糖和总多糖的含量，多糖相对分子质量及其单糖组成上均存在较大差异。

Saccharide Analysis of Abelmoschus manihot Gum

Objective:To analyze the contents of saccharides,the molecular weights of polysaccharides and monosaccharide composition of Abelmoschus manihot gum(AMG) from four different sources.Methods:The contents of reducing sugars,total saccharides and polysaccharides in four kinds of AMG samples were determined by 3,5-dinitros-alicylic acid(DNS) colorimetric method.Infrared spectra(IR) of AMG samples were also analyzed.The molecular weights of polysaccharides in purified AMG samples were characterized by gel permeat...