| 1mm内径HPLC整体柱用于蛋白质的高效分离 |
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| 引用本文： | Sebastiaan Eeltink,RemcoSwart. 1mm内径HPLC整体柱用于蛋白质的高效分离[J]. 生命科学仪器, 2010, 8(2): 21-22 |
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| 作者姓名： | Sebastiaan Eeltink RemcoSwart |
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| 作者单位： | The authors are with Dionex Corp., Abberdaan 114, 1046 AA |
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| 摘 要： | 介绍了1mm内径的聚苯乙烯-二乙烯基苯整体柱用于液相色谱反相梯度高效分离完整的蛋白质。文中详细研究了柱温、流速和梯度时间等对峰宽和峰容量的影响。在最优色谱条件下该柱高效地反相分离了核糖核酸酶A、肌红蛋白和碳酸酐酶的蛋白质混合物以及大肠杆菌提取蛋白。
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| 关 键 词： | 高效分离 蛋白质 整体柱 HPLC 内径 核糖核酸酶A 二乙烯基苯 液相色谱 |
1-mm-i.d.Monolithic HPLC Columns for High-Efficiency Protein Separations |
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| Sebastiaan Eeltink,Remco Swart. 1-mm-i.d.Monolithic HPLC Columns for High-Efficiency Protein Separations[J]. Life Science Instruments, 2010, 8(2): 21-22 |
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| Authors: | Sebastiaan Eeltink Remco Swart |
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| Affiliation: | Sebastiaan Eeltink and Remco Swart |
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| Abstract: | This article describes the LC performance of 1-mm-i.d. poly（styrene-co-divinylbenzene） monolithic columns, which were evaluated for re- versed-phase, gradient elution separations of intact proteins. The effects of column temperature, flow rate, and gradient time on peak width and peak capacity are discussed. High column efficiency at optimized LC conditions is demonstrated with the reversed-phase separation of a pro- tein sample containing ribonuclease A, myoglobin, and carbonic anhydrase and the separation of a complex mixture of intact E. coli proteins. |
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