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| 鲁西黄牛乳铁蛋白N-末端多肽基因的表达与活性检测 | |
| 引用本文： | 罗红霞,郭慧媛,林少华,陈尚武,任发政. 鲁西黄牛乳铁蛋白N-末端多肽基因的表达与活性检测[J]. 中国乳品工业, 2005, 33(12): 4-7 |
| 作者姓名： | 罗红霞 郭慧媛 林少华 陈尚武 任发政 |
| 作者单位： | 中国农业大学,食品科学与营养工程学院,北京,100083 |
| 基金项目： | 本项目受国家科技部（2002BA501A07）和北京市教委（GJSYl0090401）资助. |
| 摘 要： | 从黄牛的肝脏中提取总的DNA，根Genebank牛乳铁蛋白的cDNA序列，设计特异性引物，采用RT-PCR进行扩增，将扩增片段克隆于PGEM－Teasy vector中。测序结果表明，该片断是乳铁蛋白基因的cDNA序列，编码50个氨基酸的150bp的多肽片断，通过BamH1和ＥcoR1双酶酶切该片断，并连接到表达载体PGEX-4T1中，转化大肠杆菌BL21，进行诱导表达，蛋白电泳显示，表达成功；经纯化、胃蛋白酶酶解后，具有抗菌生物活性。 |
| 关 键 词： | 乳铁蛋白肽 克隆表达 抗菌活性 |
| 文章编号： | 1001-2230（2005）12-0004-04 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2005-11-23 |
Gene expression and activity detection of N-terminal polypeptide derived from bovine lactoferrin |
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| LUO Hong-xia,GUO Hui-yuan,LIN Shao-hua,CHEN Shang-wu,REN Fa-zheng. Gene expression and activity detection of N-terminal polypeptide derived from bovine lactoferrin[J]. China Dairy Industry, 2005, 33(12): 4-7 | |
| Authors: | LUO Hong-xia GUO Hui-yuan LIN Shao-hua CHEN Shang-wu REN Fa-zheng |
| Affiliation: | College of Food Science and Nutritional Engineering, China Agricultural University Beijing, 100083, China |
| Abstract: | A 150 bp N-terminal fragment of bovine lactoferrin was amplified by PCR with gDNA extracted from bovine liver as template.The primers were designed based on the cDNA sequence of bovine lactoferrin deposited in Genebank.The amplified fragment was cloned to expression vector and overexpressed in Escherichia coli BL21(DE3).After enzymatic proteolysis,the expressed product was used for biological function determination and antimicrobial activity was detected. |
| Keywords: | lactoferricin expression activity detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |