Evaluation of hydrogels for soft tissue adhesives <Emphasis Type="Italic">in vitro</Emphasis> and <Emphasis Type="Italic">in vivo</Emphasis> analyses |
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| Authors: | Liu Yuan Wenshuai Fan Linyingjun Han Changan Guo Zuoqin Yan Meifang Zhu Xiumei Mo |
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| Affiliation: | 1.State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering,Donghua University,Shanghai,China;2.Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology,Donghua University,Shanghai,China;3.Department of Orthopedics,Zhongshan Hospital of Fudan University,Shanghai,China;4.Shandong International Biotechnology Park Development Co., Ltd.,Yantai,China |
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| Abstract: | In this study, natural materials (sodium alginate, dextran, gelatin and carboxymethyl chitosan) were modified to get aldehyde components and amino components. Upon mixing the two-component solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and could seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution confirmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of commercial fibrin glue and the blank control. The histological staining observation by hematoxylin and eosin staining (HE) and Masson’s trichrome staining (MTC) methods suggested that the hydrogels had good biocompatibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and could improve the formation of new collagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive. |
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