| 人工培育蝉花虫草的抗肿瘤活性 |
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| 引用本文： | 陈安徽,邵 颖,李继武,蒋昭志,陈宏伟.人工培育蝉花虫草的抗肿瘤活性[J].食品科学,2015,36(9):194-197. |
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| 作者姓名： | 陈安徽 邵 颖 李继武 蒋昭志 陈宏伟 |
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| 作者单位： | 1.徐州工程学院 江苏省食品资源开发与质量安全重点建设实验室，江苏 徐州 221008；
2.亳州千草药业有限公司，安徽 亳州 236800 |
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| 摘 要： | 以天然蝉花虫草生药为对照，以中国仓鼠卵巢肿瘤（Chinese hamster ovary，CHO）细胞株为模型，采用刃天青染色法检测人工培育蝉花虫草的抗肿瘤活性。结果表明：人工培育蝉花虫草和天然蝉花虫草生药都具有抗肿瘤活性，且活性成分均为中等极性化合物。对人工培育蝉花虫草中的抗肿瘤活性成分进行分离制备，得到两种化合物，命名为化合物1和化合物2。化合物1经鉴定为虫草素，化合物2经纯度验证为纯品，活性验证结果发现当其质量浓度为50 μg/mL时，对CHO细胞抑制率高达（94.55±2.33）%。
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| 关 键 词： | 人工培育蝉花虫草 抗肿瘤活性 CHO细胞 虫草素 |
Antitumor Activity of Cultured Fruiting Bodies of Cordyceps cicadae |
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| CHEN Anhui,SHAO Ying,LI Jiwu,JIANG Zhaozhi,CHEN Hongwei.Antitumor Activity of Cultured Fruiting Bodies of Cordyceps cicadae[J].Food Science,2015,36(9):194-197. |
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| Authors: | CHEN Anhui SHAO Ying LI Jiwu JIANG Zhaozhi CHEN Hongwei |
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| Affiliation: | 1. Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology,
Xuzhou 221008, China; 2. Bozhou Qiancao Pharmaceutical Co. Ltd., Bozhou 236800, China |
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| Abstract: | The antitumor activity of the cultured fruiting bodies of Cordyceps cicadae on Chinese hamster ovary (CHO) cells
was investigated by measuring cell viability using a resazurin reduction assay in comparison with that of wild Cordyceps
cicadae. The experimental results showed that both samples had antitumor activity and contained mid-polar compounds.
Two bioactive compounds, namely components 1 and 2, were separated from the cultured fruiting bodies of Cordyceps
cicadae. Component 1 was identified as cordycepin. Component 2 (50 μg/mL) was identified as a purified compound that
could inhibit the viability of CHO cells by up to (94.55±2.33)%. |
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| Keywords: | cultivated Cordyceps cicadae antitumor activity Chinese hamster ovary (CHO) cells cordycepin |
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