| 手性色谱法测定L-乳酸锌 |
| |
| 引用本文： | 何悦铭,王 杉,揭琴丰,邱伟华,黄晓婉,邓泽元.手性色谱法测定L-乳酸锌[J].食品科学,2015,36(14):107-110. |
| |
| 作者姓名： | 何悦铭 王 杉 揭琴丰 邱伟华 黄晓婉 邓泽元 |
| |
| 作者单位： | 1.南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047；2.江西省疾病预防控制中心，江西 南昌 330029 |
| |
| 基金项目： | 2013年食品安全国家标准制定项目（spaq-2013-25） |
| |
| 摘 要： | 研究高效液相色谱法测定乳酸锌中L-乳酸锌含量的方法，并对此方法学进行探讨。采用OA-5000手性色谱柱，1 mmol/L CuSO4·5H2O溶液为流动相，流速为1 mL/min进行色谱分离DL-乳酸锌，分离效果好。L-乳酸锌在0.147～3.675 mg/mL范围内呈现良好的线性关系，相关系数达0.999 9（n=6）；精密度实验相对标准偏差为0.044%；稳定性实验相对标准偏差为0.061%；平均回收率达（99.8±1.90）%，相对标准偏差仅为1.90%。表明此方法操作简便，结果准确可靠，可用于L-乳酸锌的准确测定和品质监控。
|
| 关 键 词： | 高效液相色谱法 乳酸锌 手性 |
Methodology Validation for Determination of L-Zinc Lactate by High Performance Liquid Chromatography with A Chiral Column |
| |
| HE Yueming,WANG Shan,JIE Qinfeng,QIU Weihua,HUANG Xiaowan,DENG Zeyuan.Methodology Validation for Determination of L-Zinc Lactate by High Performance Liquid Chromatography with A Chiral Column[J].Food Science,2015,36(14):107-110. |
| |
| Authors: | HE Yueming WANG Shan JIE Qinfeng QIU Weihua HUANG Xiaowan DENG Zeyuan |
| |
| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. Center for Disease Control and Prevention of Jiangxi Province, Nanchang 330029, China |
| |
| Abstract: | This paper describes the development of a validated method for determining L-zinc lactate by high performance
liquid chromatography (HPLC). An ideal chromatographic separation between D-zinc lactate and L-zinc lactate was achieved
on an OA-5000 chiral column using 1 mmol/L CuSO4·5H2O as mobile phase at a flow rate of 1 mL/min. The established
calibration curve showed a good linearity over the concentration range of 0.147 to 3.675 mg/mL, with a correlation
coefficient of 0.999 9 (n = 6). The relative standard deviation (RSD) for precision and stability were respectively 0.044%
was and 0.061%, and the average recovery was (99.8 ± 1.90)% with RSD of 1.90%. It is shown that this method is simple,
accurate and reliable, and can be used to monitor the content of L-zinc lactate. |
| |
| Keywords: | high performance liquid chromatography (HPLC) L-zinc lactate chirality |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
