光温条件对新疆雪莲愈伤组织反应器培养后再分化能力的影响

研究了光质、光周期、昼夜温差和低温处理时间对反应器大规模培养后的新疆雪莲愈伤组织再分化能力的影响. 实验结果表明，长波长光更有利于愈伤组织的再分化. 光照时间显著影响愈伤组织的再分化能力，光照时间为16 h/d时愈伤组织的分化频率和平均出芽数达到最大，分别为76.7%和1.8个/块. 昼夜温差与愈伤组织的再分化能力呈显著负相关. 与水母雪莲不同，低温处理并不利于新疆雪莲愈伤组织的再分化. 新疆雪莲愈伤组织的再分化能力与同工酶的种类和活性密切相关.

Effects of Light and Temperature on the Redifferentiation of Saussurea involucrata Callus Cultured in the Bioreactor

A series of experiments were carded out to study the effects of light quality, light time, temperature difference between day and night, and time of low temperature treatment on the redifferentiation of Saussurea involucrata callus after the callus culture in a bioreaetor. Compared with short wavelength light, long wavelength light led to high redifferentiation. The redifferentiation frequency of callus increased with the lighting time. The maximal redifferentiation frequency was obtained with daily lighting 16 h/d. The temperature difference between day and night was negatively correlative to the callus redifferentiation. Low temperature treatment was not necessary to the redifferentiation. The peroxidase isoenzyme analysis indicated that the redifferentiation frequency of the callus was closely related to the quantity and kinds of the peroxidase isoenzymes.