黄花油点草总黄酮超声提取工艺的响应面优化及抗氧化性分析

以秦巴山区药用植物黄花油点草为研究对象,在单因素实验基础上以超声功率、提取时间、提取温度、料液比为考察因素,黄花油点草总黄酮得率为响应值,采用响应面法优化黄花油点草总黄酮的提取工艺,并采用邻二氮菲法、DPPH法对黄花油点草的不同溶剂提取物抗氧化活性进行评价。实验结果表明:黄花油点草总黄酮超声提取工艺的最佳条件为:料液比为1:40 (g/mL)、提取温度为45 ℃、超声提取时间为60 min、超声功率为210 W,在此条件下黄花油点草总黄酮得率为(12.966±0.045) mg/g,与模型预测值13.035 mg/g 相近。黄花油点草提取物对DPPH自由基、羟自由基均有作用,其中以50%乙醇提取物的抗氧化活性最好,甲醇提取物次之,水提取物最小。

Optimization of extraction technology by response surface methodology and inoxidizability of total flavonoids from Tricyrtis maculata Machride

The response surface methodology was used to optimize ultrasound-assisted extraction of total flavonoids from Tricyrtis maculata Machride.Based on single factor experiment and the extraction rate of total flavonoids for evaluation index, an ultrasonic procedure for the extraction of total flavonoids from T. maculata Machride was established by Box-Benhnken center-united experiment design and response surface analysis method. The main ultrasonic extraction factors including extraction ultrasonic power, ultrasonic time, extraction temperature, solid-liquid ratio which effect on the extraction yield of total flavonoids from T.maculata Machride were analyzed. The results showed that the optimum extraction conditions of total flavonoids from T.maculata Machride as follows:Material liquid ratio of 1:40 (g:mL), extraction temperature of 45℃, ultrasonic time of 60 min, extraction ultrasonic power of 210 W. The extraction rate of total flavonoids was (12.966±0.045) mg/g under the optimal extraction conditions and closing to predicted extraction rate of 13.035 mg/g. The results showed that the extractive of T.maculata Machride had a scavenging effect on DPPH free radical and hydroxyl radical. It had been revealed from result that the antioxidant activity of 50% ethanol extractive was the greatest, which of the methanol extractive was inferior and of the water extractive was the least.