微波辅助提取牡丹籽粕多糖工艺优化及其体外抗氧化活性

研究牡丹籽粕多糖(PA)提取工艺及其抗氧化活性。采用微波辅助法提取超临界萃取牡丹籽油后的籽粕中的多糖,探讨微波处理时间、功率、粒度和料液比对多糖提取率的影响,通过正交实验优化提取工艺,用3,5-二硝基水杨酸盐法定量分析多糖含量,用DPPH和脂质过氧化法分析牡丹籽粕多糖的抗氧化活性。结果表明,各因素对多糖提取得率的影响大小依次为固液比>微波处理功率>籽粕粒度>微波处理时间,牡丹籽粕多糖的最佳提取工艺为:微波功率480 W,提取时间8 min,粒度120目,固液比1:25 (w/v),此条件多糖得率为9.21%。牡丹籽粕多糖溶液能有效的清除DPPH自由基,抑制卵黄组织匀浆的脂质过氧化作用。体外实验牡丹籽粕多糖具有一定程度的抗氧化能力,可成为一种新的天然抗氧化剂。本研究为牡丹籽粕的综合利用提供了理论依据与参考。

Optimization of microwave-assisted extraction technology of polysaccharide from tree peony seed meal and evaluation of antioxidant activity in vitro

To develop polysaccharide by microwave-assisted extraction technique and test its antioxidant activity. The polysaccharide was extracted from tree peony seed meal, and that was determined by 3, 5-Dinitrosalicylic acid, to investigate extraction time, extraction power, seed meal granularity and the ratio of material to water. Based on these experiments, finally the orthogonal experiments were to optimize extraction condition. Meanwhile, the ability of polysaccharide to scavenge hydroxyl radical was studied to make a preliminary determination of its biological activity. The results showed the effect order of each factor on yield was as follows:stock ratio>extraction power>seed meal granularity>extraction time and the optimal extraction conditions of polysaccharide were 480 W microwave power for 8 min in combination with stock ratio 1:25 (m/v)and the size of granularity among 120 items, by which the yield of polysaccharide was up to 9.21%. The crude polysaccharide from tree peony seed meal could scavenge DPPH radical and inhibit lipid peroxidation of polyunsaturated fatty from yolk lipoprotein. Thus, the polysaccharide from tree peony seed meal could be developed as a new type of anti-oxidants. This study has provided a technical basis for further development of tree peony seed meal polysaccharide functions and utilization of tree peony resources.