天然非营养型甜味料发酵乳对四氧嘧啶诱导糖尿病模型大鼠糖代谢的影响

研究天然非营养型甜味料发酵乳对四氧嘧啶诱导糖尿病大鼠一般症状和血糖代谢的影响。添加天然非营养型甜味料、蔗糖及不添加甜味剂制备发酵乳并测定发酵乳感官和理化指标。采用四氧嘧啶腹腔注射诱导大鼠糖尿病模型,利用三种发酵乳灌胃干预4周,灌胃量为2 mL/100 g·bw·d,持续测量大鼠饮水、摄食量、体重和空腹血糖(GLU);测定糖化血红蛋白(HbA1c)、胰岛素(INS)、口服糖耐量(OGTT)。结果表明,使用天然非营养型甜味料与传统发酵乳在感官品质上无显著差异(p>0.05);灌胃4周后,各发酵乳大鼠血糖水平均恢复到20.20 mmol/L以下,胰岛素水平恢复到11.72 mIU/L以上,多饮多食和体重减轻等症状得到改善,其中利用甜味料制备的发酵乳效果更好,血糖值为18.80 mmol/L,胰岛素水平为13.85 mIU/L,口服糖耐量曲线下面积为42.30 h·mmol/L,糖化血红蛋白为10.53%,与阳性对照组(吡啶甲酸铬)无显著差异(p>0.05)。因而天然非营养型甜味料发酵乳具有一定的降糖活性。

Effect of Fermented Milk with Natural Non-nutritious Sweetener on Alloxan-induced Diabetic Rats

The aim of this work was to investigate the effect of fermented milk of natural non-nutritional sweetener on the general symptom and blood glucose metabolism of alloxan-induced diabetic rats. The fermented milk was prepared separately by adding natural non-nutritional sweetener,sucrose and without sweetener,and the sensory and physicochemical indexes of fermented milk were determined. Rat model of diabetes mellitus was induced by intraperitoneal injection of alloxan. Three kinds of fermented milk were fed by the dose of 2 mL/100 g·bw·d.Drinking water,food intake,body weight and fasting blood glucose(GLU)of rats were measured continuously,glycosylated hemoglobin(HbA1c),insulin(INS)and oral glucose tolerance(OGTT)were also assessed. The results showed that there was no significant difference in sensory quality between traditional fermented milk and the one made from natural non-nutritional sweetener(p>0.05). After 4 weeks,the blood glucose level of each group of fermented milk was restored to 20.20 mmol/L,the insulin level was restored to 11.72 mIU/L,and the symptoms of diet increase and weight loss were also alleviated. The fermented milk produced by natural non-nutritional type was more effective than the other two groups,and as effective as that of the chromium picolinate-treated group(p>0.05),the blood sugar was 18.80 mmol/L,the level of insulin was 13.85 mIU/L,the area under the oral glucose tolerance curve was 42.30 h·mmol/L and the glycated hemoglobin was 10.53%. Therefore,the fermented milk prepared by natural non-nutritional sweetener has a certain hypoglycemic activity.