测定尿液中血红蛋白含量的共振散射新方法

在酸性介质中,锌试剂和血红蛋白通过静电引力作用生成离子缔合物,从而导致体系的共振散射信号增强,据此建立了一种简单、灵敏、快速测定血红蛋白含量的共振散射光谱新方法。在优化的实验条件下,血红蛋白质量浓度在0.04~1.20μg/m L范围内与体系共振散射强度的增加值(ΔI)呈良好的线性关系(λ=605 nm),线性回归方程为ΔI=113.43ρ-0.517 0(μg/m L),相关系数r=0.998 3,方法检出限为0.02μg/m L。将该方法应用于尿样中血红蛋白含量的检测,回收率为93.40%~106.66%,结果令人满意。

A New Resonance Light Scattering Method for the Determination of Hemoglobin in the Urine

Zincon reacted with hemoglobin to form ion-association complexes through electrostatic effects in acidic medium,which greatly enhanced the resonance light scattering intensity.So a simple,sensitive and rapid method for the determination of trace amounts of hemoglobin was established.Under the optimal conditions,the increased intensity(△I) was linear to the concentration of hemoglobin in the range of 0.04～ 1.20 μg/mL at the wavelength of 605 nm,equation of linear regression △I=113.43p-0.517 0,with a correlation coefficient of 0.998 3 and a detection limit of 0.02 μ,g/mL.This method has been applied to the determination of hemoglobin in the urine samples with satisfactory results,the recoveries were in the range of 93.40％～ 106.66％.