金叶女贞果实花青素的镇痛作用初探

目的: 研究金叶女贞果实花青素的镇痛作用及其可能机制。方法: 实验动物分为5组:生理盐水对照组,阿司匹林对照组,花青素高、中、低剂量组。通过小鼠热板法和醋酸扭体法,观察不同浓度花青素的镇痛作用;同时测定小鼠血清中超氧化物歧化酶(SOD)、NO和前列腺素E₂(PGE₂)水平,初步分析花青素可能的镇痛机制。结果: 中、高浓度的花青素能提高小鼠痛阈,减少扭体次数;花青素处理组小鼠血清SOD活性增高,NO和PGE₂含量降低。结论: 金叶女贞花青素具有镇痛作用,其机制可能与提高小鼠抗氧化能力增强血清SOD活性,减少NO和炎性因子PGE₂的生成有关。

Exploration on analgesia effect of anthocyanins from Ligustrum vicaryi fruit

AIM: To investigate the analgesic effect mechanism of anthocyanins from Ligustrum vicaryi. METHODS: Experimental animals were divided into 5 groups (n=8): normal saline control group (NS);aspirin control group (Asp);high-concentration anthocyanins group (HA);medium-concentration anthocyanins group (MA);low-concentration anthocyanins group (LA). We observed the analgesia effect of different concentration anthocyanins by hot-plate test and acetic acid writhing test. The activity of superoxide dismutase (SOD) and the contents of nitric oxide (NO) and prostaglandin E₂(PGE₂) in blood serum were determined to analysis the analgesia mechanism. RESULTS: The pain threshold of mice was enhanced and the times of twist body was decreased by medium-concentration and high-concentration anthocyanins. The activity of SOD was increased and the contents of NO and PGE₂ were reduced in blood serum. CONCLUSION: Anthocyanins of Ligustrum vicaryi has definite analgesic effect, which was related to increasing the activity of SOD and decreasing the contents of NO and PGE₂.