响应面法优化微生物诱导碳酸钙沉积培养基

通过响应面法对微生物诱导CaCO3沉积的培养基组分进行优化。结果表明，最适宜培养基配方为葡萄糖30g/L、大豆蛋白胨10g/L、尿素50g/L、硝酸钙0.5mol/L、吐温80体积分数0.05%以及氯化镍250μmol/L。葡萄糖浓度和尿素浓度、葡萄糖浓度和硝酸钙浓度、葡萄糖浓度和吐温浓度、硝酸钙浓度和吐温浓度以及尿素浓度和氯化镍浓度，对微生物诱导CaCO3沉淀量的影响有着比较显著的双因子效应。沉淀物CaCO3含有少量有机质，形成方解石型或方解石和球霰石混合晶型的CaCO3，其形貌和堆积密度随外界条件改变而不同。

Optimization for microbiological-induced CaCO3 precipitation medium using response surface methodology

Response surface methodology was applied to optimize the medium composition for microorganism induced CaCO3 precipitation. The results show that the optimal medium formulation was glucose 30g/L，soybean peptone 10g/L，urea 50g/L，calcium nitrate concentration 0.5mol/L，Tween 80 concentration 0.05% and chlorination nickel 250μmol/L. The glucose concentration and urea concentration，glucose concentration and calcium nitrate concentration，glucose concentration and Tween concentration，calcium nitrate concentration and Tween concentration as well as urea concentration and chlorination nickel concentration had significantly two-factor effect on microorganism induced CaCO3 precipitation yield. Precipitated CaCO3 contained a small amount of organic matter，and the morphology and bulk density of CaCO3 on forming calcite or calcite-vaterite mixed crystal depended on precipitation conditions changes.