Limitations of the method using peroxidase activity of hemoglobin for detecting lipid hydroperoxides |
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Authors: | Shibata Selma Setsu Terao Junji Matsushita Setsuro |
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Affiliation: | (1) Research Institute of Food Science, Kyoto University, Uji, 611 Kyoto, Japan |
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Abstract: | The method using peroxidase activity of hemoglobin (Hb) for the determination of lipid peroxides was examined by using pure
methyl linoleate hydroperoxides, trilinoleoylglycerol hydroperoxides and egg yolk phosphatidylcholine hydroperoxides as substrates
and tetramethyl benzidine as electron donor for the peroxidase reaction of Hb. The reactivities of these substrates were quite
varied. Furthermore, some electron donors were tested for peroxidase activity of Hb, but none showed a complete reduction
of methyl linoleate hydroperoxides. From these results, it seems the Hb method needs to be carefully applied to biological
materials that contain mixtures of different types of lipid classes. |
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