乙肝表面抗原在微球表面的吸附行为、活性和结构变化

采用快速膜乳化技术结合溶剂挥发法制备了尺寸均一的聚乳酸(PLA)微球,平均粒径为800 nm左右,并采用PLA微球对乙肝表面抗原(HBsAg)进行了吸附研究,考察了pH值、盐浓度、微球用量、HBsAg浓度及吸附温度对HBsAg吸附率、活性和结构的影响. 结果表明,在pH 6.0的磷酸缓冲溶液中,NaCl浓度为20 g/L、微球用量为20 mg/mL、HBsAg浓度为10 mg/mL、吸附温度为37℃的条件下,HBsAg吸附率可达60%左右. 4℃下,pH值为8.0及NaCl浓度为1 g/L、微球用量为2 mg/mL及HBsAg浓度为300 mg/mL时,HBsAg活性保留可达98%以上.

Adsorption Behavior, Bioactivity and Structure of HBsAg on Surface of PLA Microspheres

Relatively uniform-sized poly(lactic acid) (PLA) microspheres with the average particle size of 800 nm were successfully prepared by premix membrane emulsification combined with solvent evaporation method. The effects of pH value, salt concentration, amount of PLA microspheres, HBsAg concentration and adsorption temperature on the adsorption efficiency, bioactivity retention and structure of HBsAg were systemically investigated. The results showed that the adsorption efficiency of HBsAg on the surface of PLA microspheres could reach more than 60% under the conditions of pH 6.0 phosphate buffer, 20 g/L NaCl concentration, 20 mg/mL PLA microspheres, 10 mg/mL HBsAg concentration and 37℃ adsorption temperature. The bioactivity retention of HBsAg exceeded 98% under 4℃ with the following conditions: pH value of phosphate buffer at 8.0, concentration of NaCl 1 g/L, amount of microspheres 2 mg/mL and initial HBsAg concentration 300 mg/mL.