离子交换法解除丁酸梭状芽孢杆菌发酵液中丁酸抑制的研究

采用离子交换法吸附发酵液中的丁酸,从18种树脂中筛选出了1种对丁酸吸交性能较好的树脂A。树脂A对10 g/L的丁酸溶液中丁酸的静态吸附量为243.3 g/kg,对5g/L丁酸梭状芽孢杆菌发酵液中丁酸的静态吸附量则为142.2 g/kg。动态吸附实验表明,树脂A吸附性能良好,洗脱峰高而窄,再生性能优良。5L发酵罐实验表明,发酵残液经陶瓷膜过滤,再经过离子交换树脂脱酸后,补充新鲜培养基打入发酵罐中进行循环使用,大大降低了原发酵液中丁酸对丁酸梭状芽孢杆菌生长的抑制,大幅度提高了菌体浓度,使之由补料分批高浓度培养时的6.1×10~8个/mL提高到1.2×10~9个/mL。

Study on Removing Inhibition of Butyric Acid from Clostridium Butyricum Fermentation Broth Using Ion-exchange Method

High-density culture must be carried out before the preparation of microecological agents.We used ion-exchange method to remove butyric acid from fermentation broth.An appropriate resin A was select- ed from more than 18 types of resins.The static state absorption capacity of Resin A was 243.3 g/kg from 5g/L butyric acid solution and 142.2g/kg from 6g/L fermentation broth.The dynamic state exchange trials, including adsorption,elute and regeneration of the resin was performed.After removing of butyric acid from fermentation broth using ion-exchange method,we studied high cell density culture with fed-batch culture in bioreactor.Cell concentration of Clostridium butyricum in 5L bioreactor high-density culture increased from 6.1×10~8个/mL to 1.2×10~9个/mL.