Quantitative and qualitative analysis of non-Saccharomyces yeasts in spontaneous alcoholic fermentations |
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Authors: | E Ocón A R Gutiérrez P Garijo C Tenorio I López R López P Santamaría |
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Affiliation: | 1. ICVV, Instituto de Ciencias de la Vid y el Vino, Universidad de La Rioja, C/Madre de Dios 51, 26006, Logro?o, Spain 2. ICVV, Instituto de Ciencias de la Vid y el Vino, Gobierno de La Rioja, C/Madre de Dios 51, 26006, Logro?o, Spain
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Abstract: | In this study, we looked at the yeast population present in four spontaneous alcoholic fermentations in the Rioja appellation
(D.O.Ca. Rioja, Spain). The study was conducted through the identification of the yeasts via the PCR–RFLP technique of the
ITS region of rDNA. In a first harvest, the qualitative diversity of the species present in spontaneous alcoholic fermentation
was studied, and in a second harvest, their quantitative significance. In spontaneous fermentations, 17 different yeast species
were found, although only two of them, Candida stellata and Kloeckera apiculata, as well as Saccharomyces cerevisiae, appeared in major proportions during fermentation. The significance of the non-Saccharomyces yeasts during the spontaneous alcoholic fermentation was conditioned by the presence of Saccharomyces cerevisiae in the medium. Species not cited in literature in connection with winemaking and yeasts associated with wines spoilage have
been detected in all the alcoholic fermentations carried out in the qualitative study. |
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