Rapid detection method for aflatoxin B1 in soybean sauce based on fluorescent microspheres probe |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China;2. Jiangxi Zodolabs Biotech Co. Ltd, Nanchang 330047, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Key Laboratory of Functional Small Organic Molecule (Ministry of Education), Jiangxi Normal University, Nanchang 330022, China;3. Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang 330047, China;4. Ocean NanoTech, LLC., San Diego, CA 92126, USA;1. College of Public Health, Zhengzhou University, Zhengzhou 450001, China;2. Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China;3. College of Grain Oil and Food Science, Henan University of Technology, Zhengzhou 450052, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Jiangxi Province Center for Disease Control and Prevention, Nanchang 330047, China;3. School of Food, Nanchang University, Nanchang 330047, China;1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;2. College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Soybean sauce, a Chinese traditional and daily condiment, is often contaminated by aflatoxin B1. An extract-free immunochromatographic assay was proposed based on fluorescent microspheres probe for the' detection of aflatoxin B1 in soybean sauce. The probe was prepared by coupling fluorescent microspheres with anti-aflatoxin B1 antibody by the 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride-mediated method. The background from the soybean sauce sample on strip was eliminated because of the optical property of the probe. The sample without extracting procedure was directly detected by diluting with 10% methanol solution. The visible detection limit for the qualitative analysis of aflatoxin B1 in the proposed method was 2.5 μg/L, which was lower than the maximum level of 5 μg/L set by the Chinese government. The results were well agreed with those obtained by liquid chromatography-mass spectrometry (LC-MS). The method showed satisfactory characteristics, such as rapid detection, easy operation, and high sensitivity, and can thus be applied for the large-scale and on-site screening of soybean sauce contaminated with aflatoxin B1. To our knowledge, this report is the first one on the qualitative detection of aflatoxin B1 in dark colored food samples directly by fluorescent microspheres probe-based immunochromatography. |
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| Keywords: | Fluorescent microspheres probe Immunochromatography Soybean sauce Detection |
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