Comparative study of conductometric glucose biosensor based on gold and on magnetic nanoparticles |
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| Authors: | Wided Nouira Abderrazak Maaref Hamid Elaissari Francis Vocanson Maryam Siadat Nicole Jaffrezic-Renault |
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| Affiliation: | 1. Université de Lyon, Institut des Sciences Analytiques: Université Claude Bernard, Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France;2. Laboratoire des Interfaces et Matériaux Avancés, Université de Monastir, Avenue de l''Environnement, 5019 Monastir, Tunisia;3. Université de Lyon, Laboratoire LAGEP CPE-308G: Université Claude Bernard Lyon-1, 69622 Villeurbanne Cedex, France;4. Université de Lyon, Laboratoire Hubert Curien, Université de Saint-Etienne Jean-Monnet, F-42023 Saint-Etienne, France;5. LASC, ISEA, Université de Metz, 7 rue Marconi, 57070 Metz, France |
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| Abstract: | The aim of this study was to show the feasibility and the performances of nanoparticle biosensing. A glucose conductometric biosensor was developed using two types of nanoparticles (gold and magnetic), glucose oxidase (GOD) being adsorbed on PAH (poly(allylamine hydrochloride)) modified nanoparticles, deposited on a planar interdigitated electrode (IDEs). The best sensitivities for glucose detection were obtained with magnetic nanoparticles (70 μM/mM and 3 μM of detection limit) compared to 45 μM/mM and 9 μM with gold nanoparticles and 30 μM/mM and 50 μM with GOD directly cross-linked on IDEs. When stored in phosphate buffer (20 mM, pH 7.3) at 4 °C, the biosensor showed good stability for more than 12 days. |
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