| 回流法提取银杏叶总黄酮的研究 |
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| 引用本文： | 李新舟,张怀宇,许友玲.回流法提取银杏叶总黄酮的研究[J].化学与生物工程,2014(3):31-34. |
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| 作者姓名： | 李新舟 张怀宇 许友玲 |
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| 作者单位： | 福建师范大学闽南科技学院生命科学与化学系,福建泉州362332 |
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| 基金项目： | 福建省教育厅科技项目（JB10214） |
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| 摘 要： | 研究了乙醇回流法提取银杏叶总黄酮的工艺条件。通过单因素实验考察料液比、乙醇体积分数、提取时间、提取温度对银杏叶总黄酮提取率的影响,通过正交实验确定优化的提取工艺条件为:料液比1∶45(g∶mL)、乙醇体积分数50%、提取时间2.0h、提取温度70℃,在此条件下,银杏叶总黄酮提取率为0.918%。以优化的工艺条件为基础进一步运用微波辅助提取银杏叶总黄酮,发现在微波功率为640W时,采用间歇式提取,提取时间为15×10s,总黄酮提取率达到0.91%,接近传统方法提取3h的提取率。
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| 关 键 词： | 银杏 总黄酮 回流法 微波辅助 间歇式提取 |
Study on Extraction of Total Flavonoids from Ginkgo Biloba Leaves by Reflux Method |
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| Affiliation: | LI Xin-zhou,ZHANG Huai-yu,XU Yon-ling( 1.Department of Life Science and Chemistry ,Minnan Science ＆ Technology Institute,Fujian Normal University, Quanzhou 362332, China;) |
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| Abstract: | Total flavonoids were extracted from Ginkgo biloba leaves by ethanol using refulx method.The effects of solid-liquid ratio ethanol volume fraction,extraction time and extraction temperature on total flavonoids extraction rate were explored by single factor experiment and orthogonal experiment.The optimal extraction conditions were determined as follows：solid-liquid ratio of 1 ∶ 45（g：mL）,ethanol volume fraction of 50 ％,extraction time of 2.0 h,extraction temperature of 70 ℃,under which total flavonoids extraction rate was up to 0.918％.Furthermore,the microwave-assisted extraction was carried out.It was found that by microwave-assisted intermittent extraction with power of 640 W,time of 15 × 10 s,the total flavonoids extraction rate reached 0.91％,approaching that obtained by traditional extraction for 3 h. |
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| Keywords: | Ginkgo biloba total flavonoids reflux method microwave-assisted intermittent extraction |
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