Selective determination of urea using urease immobilized on ZnO nanowires |
| |
Authors: | Syed M Usman Ali Zafar Hussain Ibupoto Salah Salman Omer Nur Magnus Willander Bengt Danielsson[Author vitae] |
| |
Affiliation: | aPhysical Electronics and Nanotechnology Division, Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174 Norrköping, Sweden;bAcromed Invest AB, Magistratsvägen 10, SE-22643 Lund, Sweden |
| |
Abstract: | Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50–130 nm diameters and ∼1 μm in lengths were used in an enzyme-based urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two linear sensitivity regions were observed when the electrochemical responses (EMF) of the sensors were plotted vs. the logarithmic concentration range of urea from 0.1 mM to 100 mM. The proposed sensor showed a sensitivity of 52.8 mV/decade for 0.1–40 mM urea and a fast response time less than 4 s was achieved with good selectivity, reproducibility and negligible response to common interferents such as ascorbic acid and uric acid, glucose, K+ and Na+ ions. |
| |
Keywords: | ZnO-nanowire arrays Electrochemical nanodevices Urease enzyme Urea determination |
本文献已被 ScienceDirect 等数据库收录! |
|