放线菌扫描电镜样品制备方法比较研究

目的:比较和筛选放线菌扫描电镜样品制备方法。方法:通过培养获得插片、菌饼和发酵液3种待处理样品,根据单因素试验设计原则采用双固定、单固定、磷酸缓冲液清洗、生理盐水清洗、蒸馏水清洗、丙酮脱水、乙醇脱水和直接表面镀金等8种方法对样品进行处理。结果:取样时间直接影响观察样品形态的丰富度;插片样品扫描获得的结果较为理想;不同的固定、清洗和脱水等处理过程也对样品的细节产生了影响。结论:插片样品直接干燥喷金扫描观察是一种简便、快速的方法,对于常规的放线菌观察与鉴定较为实用,也为其它微生物扫描电镜观察提供了参考。

Comparison of preparation method for actinomyces scanning electron microscopy sample

Objective：To compare and screening actinomycetes scanning electron microscopy sample preparation method. Methods：Eight kinds of processing method（ dual fixed, single fixed, washed by phosphate buffer, saline, distilled water, dehydrated by acetone, ethanol, surface plating directly） for processing the samples based on single-factor test designing principles. Results： Sampling time had a direct impact on the morphology richness of the observed sample; insert samples obtained more perfect results; no significant difference between two fixing methods;the samples of different sources should use different cleaning methods;there was no significant difference between the two dehydrating methods. Conclusion：Inserting sample dried directly, sprayed gold and scaned was a reliable, simple, rapid method and was more practical for conventional observetion and identification of actinomycetes and provided a reference for the scanning electron microscope observation of other microorganisms.