Comparison of candicidin, levorin and trichomycin by means of high-performance liquid chromatography

A high-performance liquid chromatographic method for the separation of individual components of polyene macrolide antibiotics has been developed. The three heptaene macrolide antibiotics, candicidin, levorin and trichomycin, have been investigated and compared. In all instances these compounds proved to be complex mixtures. It is demonstrated that candicidin and levorin are identical, whereas trichomycin differs in composition from these two substances.