超导体包被免疫荧光试纸法同时测定玉米中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮适用性研究

为了能同时快速测定玉米中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和玉米赤霉烯酮(Zearalenone,ZEN)两种毒素,采用基于超导体包被的免疫荧光快速定量检测技术,检测了多个玉米样品,结合加标回收率、稳定性、检出限、精密度等指标,并与液相色谱法的检测结果进行比较分析,评估方法的适用性。结果表明,超导体包被的免疫荧光试纸法检测DON含量在100～2 500μg/kg,检测ZEN含量在5～200μg/kg的范围内线性良好。DON在500～2 000μg/kg添加水平范围内,回收率为103.72%～108.17%,ZEN在50～150μg/kg添加水平范围内,回收率为97.81%～111.27%。该方法于液相色谱法检测结果对比,DON相对偏差在3.72%～8.17%,ZEN相对偏差在2.19%～11.27%,均低于液相色谱法允许偏差23%和15%,超导体包被的免疫荧光试纸法是一种有效、实用、快速、定量的分析方法,能满足同时检测玉米中DON和ZEN的要求。

Research on applicability of superconductor coated immunofluorescence test strip method for determination of deoxynivalenol and zearalenone in maize(Online First, Recommended Article)

In order to rapidly determine deoxynivalenol (DON) and Zearalenone (ZEN) in maize, a rapid quantitative immunofluorescence assay based on superconductor coating was used to detect multiple maize samples. Combined with the recovery rate, stability, detection limit, precision and other indexes, the applicability of the method was evaluated and compared with the results of liquid chromatography. The results showed that the superconductor coated immunofluorescence diaper method had the good linearity in the range of 100 ng/mL~2 500 ng/mL for DON and 5~200 µg/kg for ZEN. The recoveries of DON were 103.72%~108.17% in the range of 500~2 000 µg/kg, and 97.81%~111.27% in the range of ZEN 50~150 µg/kg. Compared with the results of liquid chromatography, the superconductor coated immunofluorescence diaper method is an effective, practical, rapid and quantitative analysis method within the allowable deviation range, which can meet the requirements of simultaneous detection of DON and ZEN in maize.