破壁油菜花粉的抗氧化及抗衰老作用

目的:探讨破壁油菜花粉对D-半乳糖所致衰老模型小鼠的抗氧化及抗衰老作用。方法:将48只小鼠随机分为空白对照组、衰老模型组、破壁油菜花粉高（560 mg/kg）、中（280 mg/kg）、低（140 mg/kg）组及V_C组。空白对照组每日腹腔注射生理盐水，其余各组每日腹腔注射D-半乳糖（120 mg/kg）诱导衰老模型，同时药物组灌胃高、中、低剂量破壁油菜花粉混悬液及V_C溶液，每周称重，连续6周后取小鼠的肝、脾、肾、胸腺称取脏器质量并计算脏器指数；生化法测定血清及肝脏中超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的含量；HE染色观察衰老小鼠肝脏形态的改变；Western Blot检测衰老小鼠肝脏组织中Bax、Sirt1、p53蛋白表达。结果:与衰老模型组相比，破壁油菜花粉中、高剂量组能显著提高衰老小鼠体质量、胸腺、肝脏、脾、肾脏的脏器指数（P<0.05，P<0.01），显著升高小鼠血清及肝脏内SOD、CAT、GSH-Px含量，降低MDA含量（P<0.05，P<0.01），同时可以改善肝细胞肿大，使得肝脏组织中细胞凋亡蛋白Bax、促衰老蛋白p53的表达降低，抗衰老蛋白Sirt1的表达升高（P<0.05，P<0.01）。结论:破壁油菜花粉可通过抗氧化，对抗细胞凋亡，调节衰老蛋白含量改善机体衰老。

Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen

Objective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) group and V_C group. The blank control group was intraperitoneally injected with normal saline every day, and the other groups were intraperitoneally injected with D-galactose (120 mg/kg) every day to induce the aging model. At the same time, the drug group was gavaged with high, medium and low doses of broken wall rape pollen suspension and V_C solution, weighed every week, and the liver, spleen, kidney and thymus of mice were taken after 6 weeks to weigh the organ mass and calculate the organ index. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-PX) in serum and liver were determined by biochemical method. HE staining was used to observe the morphological changes of liver in aging mice. The expressions of Bax, Sirt1 and p53 proteins in the liver of aging mice were detected by Western Blot. Results: Compared with the aging model group, the medium and high dose groups of broken wall rape pollen could significantly improve the body mass, organ index of thymus, liver, spleen and kidney (P<0.05, P<0.01), significantly increase the contents of SOD, CAT and GSH-Px in serum and liver, reduce the content of MDA (P<0.05, P<0.01), improve hepatocyte swelling, make the expression of the apoptotic protein Bax and pro-aging protein p53 decrease, and the expression of anti-aging protein Sirt1 increase (P<0.05, P<0.01). Conclusion: Broken wall rape pollen can improve the aging of the body by anti-oxidation, anti apoptosis, regulating the content of aging protein.